产品货号：
Z15692
中文名称：
Collagen Type V抗体
英文名称：
Anti-Collagen Type V Antibody
产品规格：
100μg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Collagen Type V Antibody |
| 指标别名 | EDSCL1;FMDMF;Collagen Type V;COL5A1 protein;Collagen fetal membrane A polypeptide;Collagen alpha-1 (V) chain;EDSC;EDSCL2;Collagen alpha-3(V) chain;Collagen alpha-2(V) chain |
| 克隆性 | Polyclonal |
| 检验物种 | bovine,human |
| 应用范围 | Dot blot,IHC |
| 基因名称 | COL5A1/COL5A2/COL5A3 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | Collagen Type V from human and bovine placenta |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | Anti-Collagen Type V has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens,human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed,paraffin embedded tissues.?This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I,II,III,IV or VI collagens.Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
| 浓度 | 1.0mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at 4℃ prior to opening.This product is stable at 4℃ as an undiluted liquid.Dilute only prior to immediate use.For extended storage,mix with an equal volume of glycerol,aliquot contents and freeze at -20℃ or below.Avoid cycles of freezing and thawing.Expiration date is one (1) year from date of opening. |
| 背景资料 | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure.For these reasons,it is often extremely difficult to generate antibodies with specificities to collagens.The development of ＆type＊ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes.Biorab extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation.This preparation results in a native conformation of the protein.Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies.Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. |
| Uniprot ID | P20908/P05997/P25940 |
| 推荐稀释比 | ELISA:1:5,000 - 1:50,000 IHC:1:50 - 1:200 IP:1:100 WB:1:1,000 - 1:10,000 |
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